The detection of Mycobacterium tuberculosis using in vitro granuloma tuberculosis model intracelluler specimens

  • Imam Agus Faizal STIKES Al-Irsyad Al-Islamiyyah Cilacap
  • Jusak Nugraha Dr. Soetomo General Hospital
  • Agung Dwi Wahyu Widodo Dr. Soetomo General Hospital
Keywords: Granuloma,Tuberculosis, Mycobacterium tuberculosis, Ziehl-Neelsen.


The Granulomas are a group of innate and adaptive immune cells that are highly organized, and during tuberculosis infection caused by the bacterium Mycobacterium tuberculosis. Estimated as many as 1.6 million cases of death in 2017 according to World Health Organization. Tuberculosis ranks in the top 10 causes of death worldwide and it is predicted that more than 1.7 billion people (around 25 percent of the world's population) are estimated to be infected by Mycobacterium tuberculosis. The purpose of this study was to confirm the Mtb by examining a new method by modifying Ziehl-Neelsen stain using an intracellular specimens for tuberculous granuloma in vitro.  This study used the isolate of the bacterium Mycobacterium tuberculosis H37Rv obtained from the Microbiology Laboratory of the Institute of Tropical Disease Universitas Airlangga, Surabayaa, using Peripheral Blood Mononuclear Cells to make model granuloma tuberculosis.  The result is Ziehl-Neelsen staining was modified, it was found that the Mycobacterium tuberculosis H37Rv as detection of bacterial confirmation is said to be successful.


Download data is not yet available.

Author Biography

Imam Agus Faizal, STIKES Al-Irsyad Al-Islamiyyah Cilacap

Kaprodi Sarjana Terapan Teknologi Laboratorium Medis

STIKES Al-Irsyad Al-Islamiyyah Cilacap


J.A. G-C, Lamo-Sevilla, De C, et al. Global Tuberculosis Research And Its Future Prospects. Pre-proof. Published online 2020:101917. doi:10.1016/

Levillain F, Kim H, Woong K, et al. Preclinical assessment of a new live attenuated Mycobacterium tuberculosis Beijing-based vaccine for tuberculosis. Vaccine. 2019;(xxxx). doi:10.1016/j.vaccine.2019.11.085

Faizal IA, Dwi A, Widodo W, Nugraha J. The Effect of Macrophage Dose to Secretion Interleukin 6 ( IL-6 ) on Model of Tuberculosis Granuloma In Vitro. In: Proceedings of the 2nd International Conference Postgraduate School (ICPS). Vol 6. Science and Technology Publications; 2018:306-311. doi:10.5220/0007541803060311

Baliga S, Murphy C, Sharon L, et al. Rapid method for detecting and differentiating Mycobacterium tuberculosis complex and non-tuberculous mycobacteria in sputum by fluorescence in situ hybridization with DNA probes. Int J Infect Dis. 2018;75:1-7. doi:10.1016/j.ijid.2018.07.011

Heemskerk AD, Donovan J, Dang D, et al. Improving the microbiological diagnosis of tuberculous meningitis : A prospective , international , multicentre comparison of conventional and modified Ziehl – Neelsen stain , GeneXpert , and culture of cerebrospinal fluid. J Infect. 2018;77(6):509-515. doi:10.1016/j.jinf.2018.09.003

Harding JS, Herbath M, Chen Y, et al. VEGF-A from Granuloma Macrophages Regulates Granulomatous Inflammation by a Non-angiogenic Pathway during Mycobacterial Infection Article VEGF-A from Granuloma Macrophages Regulates Granulomatous Inflammation by a Non-angiogenic Pathway during Mycobacteri. CellReports. 2019;27(7):2119-2131.e6. doi:10.1016/j.celrep.2019.04.072

Suryana K, Suharsono H, Antara J, Rai IBN. Effect of isoniazid preventive therapy on pulmonary tuberculosis activity in people living with hiv/aids at wangaya hospital in Denpasar, Bali, Indonesia: A prospective cohort study. Bali Med J. 2020;9(1):219-223. doi:10.15562/bmj.v9i1.1684

LI Q, ZHAO YL, WANG Q, et al. Evaluation of the New Automatic Mycob.T Stainer and Scanner for Detecting Acid-fast Bacilli in China. Biomed Environ Sci. 2018;31(8):572-578. doi:10.3967/bes2018.078

Chen P, Shi M, Feng G, et al. A Highly Efficient Ziehl-Neelsen Stain : Identifying De Novo Intracellular Mycobacterium tuberculosis and Improving Detection of Extracellular M . tuberculosis in Cerebrospinal Fluid. J Clin Microbiol. 2012;4:1166-1170. doi:10.1128/JCM.05756-11

Karimi S, Shamaei M, Pourabdollah M, et al. Histopathological Findings in Immunohistological Staining of the Granulomatous Tissue Reaction Associated with Tuberculosis. Tuberc Res Treat. 2014;2014:1-6. doi:10.1155/2014/858396

Ağel E, Sağcan H, Ceyhan İ, Durmaz R. Optimization of isothermal amplification method for Mycobacterium tuberculosis detection and visualization method for fieldwork. Turkish J Med Sci. 2020;50:1069-1075. doi:10.3906/sag-1910-6

Basu A, Chakrabarti I, Ghosh N, Chakraborty S. A clinicopathological study of tuberculous pleural effusion in a tertiary care hospital. Ann Trop Med Public Heal. 2012;5(3):1-6. doi:10.4103/1755-6783.98606

Prasad CSBR, Narasimha A, Kumar MLH. Negative staining of mycobacteria – A clue to the diagnosis in cytological aspirates : Two case reports. Ann Trop Med Public Heal. 2011;4(2):110-112. doi:10.4103/1755-6783.85763

Ndlovu H, Marakalala MJ. Granulomas and inflammation: Host-directed therapies for tuberculosis. Front Immunol. 2016;7(OCT). doi:10.3389/fimmu.2016.00434

Majeed S. Dual Role of Inflammation in Prognosis and Prevention of Tuberculosis. J Clin Cell Immunol. 2015;6(1):1-9. doi:10.4172/2155-9899.1000298

Guirao JJ, Cabrera CM, Jiménez N, Rincón L, Urra JM. High serum IL-6 values increase the risk of mortality and the severity of pneumonia in patients diagnosed with COVID-19. Mol Immunol. 2020;128(October):64-68. doi:10.1016/j.molimm.2020.10.006

Alves DA, Silva M V, Barros CCO, et al. TNF- α blockade impairs in vitro tuberculous granuloma formation and down modulate Th1 , Th17 and Treg cytokines. PLoS One. 2018;13(3):1-13. doi:10.1371/journal.pone.0194430

Cicchese JM, Dartois V, Kirschner DE, Linderman JJ, Salomone S. Both Pharmacokinetic Variability and Granuloma Heterogeneity Impact the Ability of the First-Line Antibiotics to Sterilize Tuberculosis Granulomas. 2020;11(March):1-15. doi:10.3389/fphar.2020.00333

Hauling T, Qian X, Kramnik I, Nilsson M, Rottenberg ME. Spatial and temporal localization of immune transcripts defines hallmarks and diversity in the tuberculosis granuloma. Nat Commun. 2019;10(4):1-15. doi:10.1038/s41467-019-09816-4

Andersson B, Jonsson M, Welin A, Lerm M, Sch T. A novel mycobacterial growth inhibition assay employing live-cell imaging of virulent M . tuberculosis and monitoring of host cell viability. 2020;124(August). doi:10.1016/

Sia JK, Rengarajan J. HHS Public Access. Dep Heal Hum Serv. 2019;7(4):3-22. doi:10.1128/microbiolspec.GPP3-0022-2018

Yuan T, Sampson NS. Hit Generation in TB Drug Discovery : From Genome to Granuloma. Published online 2018. doi:10.1021/acs.chemrev.7b00602

Zaharie SD, Franken DJ, Kuip M Van Der, et al. The immunological architecture of granulomatous inflammation in central nervous system tuberculosis. Tuberculosis. Published online 2020:102016. doi:10.1016/

PlumX Metrics

How to Cite
Faizal, I. A., Nugraha, J., & Widodo, A. D. W. (2020). The detection of Mycobacterium tuberculosis using in vitro granuloma tuberculosis model intracelluler specimens. Medical Laboratory Analysis and Sciences Journal, 2(1), 16-22.
Abstract viewed = 130 times FULL TEXT downloaded = 96 times